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留言内容：QuantStudio 6 Flex Real-Time PCR System
The Applied Biosystems(TM) QuantStudio(TM) 6 Flex Real-Time PCR System is ideal for laboratories with multiple applications and end users on a limited budget. With a planned upgrade path to a Applied Biosystems(TM) QuantStudio(TM) 7 Flex System that accommodates automation or TaqMan Array Cards, the QuantStudio 6 Flex System is an ideal qPCR platform to accommodate changing future needs.
QuantStudioTM 6 Flex实时荧光定量PCR系统是开展多种应用及的终端用户的理想选择。QuantStudioTM 6 Flex系统可通过预先设计好的途径升级至QuantStudioTM 7 Flex系统，而后者能够胜任自动化操作或兼容TaqMan(R)微流体芯片，因此QuantStudioTM 6 Flex系统是能够适应用户不断变化的需求的理想qPCR平台。
先期投入最小化 只需一台仪器，您即可在96孔、快速96孔与384孔形式间切换。
跳过复杂的学习过程
直观的软件，简单的触摸屏设置以及方便的模块更换操作，均为助您即刻上手而设计。
实现灵敏度和性能的完美统一 在单重反应中检测到低至1.5倍的变化，实现10倍对数动态范围。
可随时根据需要升级 QuantStudio(TM) 6 Flex系统能够由我们的现场服务工程师无缝升级至QuantStudio(TM) 7 Flex系统，从而增加自动化、通量和多重分析能力。
QuantStudio(TM)
QuantStudio(TM)
QuantStudio(TM)
QuantStudio(TM)
6 (21 filter combinations)
6 (21 filter combinations)
2 (endpoint detection)
SYBR(R), TaqMan(R)
SYBR(R), TaqMan(R)
SYBR(R), TaqMan(R)
TaqMan(R) Only
TaqMan(R) Array Card
(384-well microfluidic card)
TaqMan(R) Array Card
(384-well microfluidic card)
OpenArray(R) plates(3,072 through-holes)
20,000 partitions/chip
Automation
Throughput
Block change from front in less than 1 minute, no tools required
enablement
Optional software module available
monitoring
Available to monitor up to 15 networked instruments simultaneously
Intuitive touch
applications
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Pharmacogenomics
Digital PCR
Molecular standards quantification
Absolute quantification
Pathogen detection
Load determination
Copy number variation
Digital PCR
See product list below
The QuantStudio(TM) 6 Flex Real-Time PCR System is supported with:
Professional installation
Premium training package conducted by field scientists
Worldwide application, technical, and field-training support
AB(R) Complete
AB(R) Assurance
AB(R) Maintenance
Repair response time (business days)
Guaranteed next-day,on-site repairs
Guaranteed 2-day on-site repairs
Targeted 2 day remedial repairs
Remote instrument monitoring and diagnostics
Priority phone and email access to instrument
Instrument operating software upgrades
Instrument qualification services
Planned maintenance
Parts, labor, and travel
Discounted with the Plus plan
先期投入最小化 只需一台仪器，您即可在96孔、快速96孔与384孔形式间切换。
跳过复杂的学习过程
直观的软件，简单的触摸屏设置以及方便的模块更换操作，均为助您即刻上手而设计。
实现灵敏度和性能的完美统一 在单重反应中检测到低至1.5倍的变化，实现10倍对数动态范围。
可随时根据需要升级 QuantStudio(TM) 6 Flex系统能够由我们的现场服务工程师无缝升级至QuantStudio(TM) 7 Flex系统，从而增加自动化、通量和多重分析能力。
QuantStudio(TM)
QuantStudio(TM)
QuantStudio(TM)
QuantStudio(TM)
6 (21 filter combinations)
6 (21 filter combinations)
2 (endpoint detection)
SYBR(R), TaqMan(R)
SYBR(R), TaqMan(R)
SYBR(R), TaqMan(R)
TaqMan(R) Only
TaqMan(R) Array Card
(384-well microfluidic card)
TaqMan(R) Array Card
(384-well microfluidic card)
OpenArray(R) plates(3,072 through-holes)
20,000 partitions/chip
Automation
Throughput
Block change from front in less than 1 minute, no tools required
enablement
Optional software module available
monitoring
Available to monitor up to 15 networked instruments simultaneously
Intuitive touch
applications
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Gene expression
miRNA profiling
SNP genotyping
Copy number variation
Protein thermal shift
High resolution melt
Pathogen detection
Pharmacogenomics
Digital PCR
Molecular standards quantification
Absolute quantification
Pathogen detection
Load determination
Copy number variation
Digital PCR
See product list below
The QuantStudio(TM) 6 Flex Real-Time PCR System is supported with:
Professional installation
Premium training package conducted by field scientists
Worldwide application, technical, and field-training support
AB(R) Complete
AB(R) Assurance
AB(R) Maintenance
Repair response time (business days)
Guaranteed next-day,on-site repairs
Guaranteed 2-day on-site repairs
Targeted 2 day remedial repairs
Remote instrument monitoring and diagnostics
Priority phone and email access to instrument
Instrument operating software upgrades
Instrument qualification services
Planned maintenance
Parts, labor, and travel
Discounted with the Plus plan
Powerful qPCR analysis tools
Applied Biosystems(TM) analysis modules make it& easier and faster to view, analyze, remotely access, store, and share qPCR data. Discover Standard Curve, Genotyping, and Relative Quantification analysis apps now.
仅限研究用途，不可用于诊断操作。
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赛默飞世尔科技（中国）有限公司地址(中国总部)：上海市浦东新区新金桥路27号3& 6& 7号楼
电话：400-650-5118 & 800-810-5118QuantStudio 3 and QuantStudio 5 Real-Time PCR SystemsConnect to your data anytime, anywhereConnect with your futureThe modern laboratory is undergoing a technological revolution. Today’s scientific breakthroughs emerge in a context of unparalleled connectivity. Lab instruments not only are more compact, automated, powerful, and accessible, but also can store and can connect to platforms that can share vast amounts of data, facilitating scientific advances through global collaboration. As your trusted partner at the leading edge of this era of innovation, we’ve developed the Applied Biosystems QuantStudio 3 and 5 Real-Time PCR Systems. These high-performance benchtop instruments allow you? ?to remotely monitor your runs, as well as easily access and securely share results with colleagues anywhere, anytime when connected to Thermo Fisher Cloud. With your data always within reach and shareable, the answers shaping the future of science are never far away.1 QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems2Discover the QuantStudio 3 and 5 Real-Time PCR SystemsThe QuantStudio 3 and 5 Real-Time PCR Systems are the latest additions to our family of QuantStudio?systems. These instruments provide our latest advancements in touch screen usability, allowing you to stayInteractivity? ? ?connected to your data easily. They’re designed for both new and experienced users who need simple and affordable real-time PCR systems without compromising performance or quality.Interactive touch screen. Run and edit directly from touch screen. Easy and intuitive interface.Access, analyze, and share data anytime, anywhere ― Remotely monitor your runs, analyze sophisticated data sets in minutes, store data in a secure space, and share results online with colleagues across institutions and around the world, with Web browserCbased software. Obtain results you can trust ― Detect differences in target quantity as small as 1.5-fold in singleplex reactions, and obtain 10 logs of linear dynamic range. Establish standard operating procedures and compliance with ease ― Locked protocol templates, in-run quality control (QC) feedback, and QC traceability of consumables offer greater control of experiment data. Real-time data mark-up language (RDML) export is available for compatibility with MIQE guidelines. Helps save valuable time ― 3 or 6 independent temperature zones for flexibility to run multiple experiments simultaneously. Fast thermal cycling is also available, enabling results in less than 30 minutes. Get started quickly ― Instrument is factory-calibrated for optical and thermal accuracy, quick installation, and immediate use. Skip the learning curve ― With preoptimized protocol templates, training is minimized for new users, allowing you to focus on your research. Maximize benchtop space ― Compact instrument can be configured as stand-alone or with a computer to fit most laboratory needs. Get a premium instrument at an a?ordable price ― Innovation doesn’t have to come at a premium price. Get the state-of-the-art Applied Biosystems functionality and industrial design that you’ve come to know, with the QuantStudio family of instruments.?Accessibility??Collaboration??Access experiment runs from any location, anytime, with remote monitoring.?Quickly share data sets and protocols online.Wi-Fi enabled connectivity.?? Send large files securely around campus or around the world.Utilize portable devices to quickly analyze data when you need to.?With Web access and Thermo Fisher Cloud.Integrate and analyze multiple data sets and data types into one project.?3QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems4InteractivitySimple, intuitive software ― at your fingertipsAccessibilityAccess QuantStudio Design and Analysis Software two ways? Interactive touch-screen interface and simplified QuantStudio Design and Analysis Software make it easy to get started and stay organized.?? Easily identifiable icons guide you through the workflow to set up runs and analyze experiments. ? Graphical interface allows easy editing of experimental conditions (Figure 1A). ? Interactive touch screen allows you to manipulate view to a particular graph or data point (Figure 1B). ? Option to pause a real-time PCR run on demand. ? Preoptimized protocol templates allow quick selection of default protocols for standard applications. ? Locked workflow feature allows for experiment consistency in tightly controlled environments. Access with Web browserCbased software Web-based or online: ? Web browserCbased system configuration with PC or Mac computers.?Co-locate with computer Desktop: ? Traditional co-located computer system configuration. ? Streamlined software for improved usability and analysis response time. ? No additional fees.? Streamlined software for improved usability and analysis response time. ? Enables secure access of your data when and where you want it. ? No software to install, no additional fees, and no versions to update. ? Monitor and check instrument status.ABFor more information about the Thermo Fisher Cloud platform and data security, go to thermofisher.com/thermofishercloudFigure 1. Graphical interface allows (A) easy editing of thermal cycling conditions and viewing of plate layout, as well as (B) viewing of amplification plots and drilling down to a subset of sample wells.5QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems6CollaborationFast and powerful secondary analysis software to extract and share resultsApplied Biosystems Analysis Modules are innovative cloud-based data analysis applications that bring together multiple data sets in one convenient place, and render them in stunning data visualizations for enhanced analysis and insights.?The Applied Biosystems Analysis Modules include:Absolute quanti?cationFigure 2. Absolute quantification module for gene expression analysis. The module enables analysis of genes of interest with the use of a standard curve. Additional ?exibility is achieved by importing standard curves from other experiments.Relative quanti?cationFigure 3. Relative quantification module for gene expression analysis. With this module, you can customize groupings of data within projects for a thorough comparison of data. The module also includes integrated correlation, volcano, and cluster plot analysis, with the ability to drill down to amplification plots.Anywhere, anytime access Access your data with a compatible browser on any device. Each registered user has a PIN-protected account on Thermo Fisher Cloud. Fast and powerful analysis Analysis speeds up to 10 times faster than our desktop software version, to help analyze more data and gain insights more quickly than before using Thermo Fisher Cloud.Easy to use One-click quality checks and comparisons between different visualizations, for simple and convenient data analysis. Integrated analysis solution Integrate your experiments into a single project ― analyze various groups of data, such as time course experiments or cell line comparisons, and pick ideal settings to easily compare data.Superior security Powered by Amazon Web Services , the Thermo Fisher Cloud platform helps protect your data in a highly secure environment using 256-bit encryption and physical security measures.?GenotypingMIQE guideline support The instrument software allows users to save predefined analysis settings for auto-exporting run data into their format of choice, including RDML (real-time data mark-up language, compliant with MIQE guidelines) export format.Figure 4. Genotyping analysis module. This module expands on existing TaqMan Genotyper software with improved visuals and integrated traces of allelic discrimination plots. The module allows for thorough quality control of SNP assays to accurately re?ect true signals vs. background noise.? ?7QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems8Generate high-quality data for a variety of applicationsPerformance you can trust Utilizing proven OptiFlex technology and VeriFlex Blocks, QuantStudio 3 and 5 systems offer improved data accuracy and sensitivity for a broad range of genomic applications, such as analyses of gene expression, microRNAs and noncoding RNAs, SNP genotyping, copy number variation, mutation detection, drug metabolism enzymes, and protein expression.? ?Assay ?exibility to support your application The QuantStudio 3 and 5 systems support probe-based assays as well as intercalating dyes. TaqMan probe-based assays, developed with powerful algorithms and optimized master mixes, enable outstanding specificity and sensitivity. SYBR Green chemistry is an economical alternative for target identification or initial screening assays. The QuantStudio 3 system has 4 filters calibrated for FAM /SYBR Green, VIC /JOE , NED /TAMRA , and ROX dyes. The QuantStudio 5 System offers 96-well and 384-well format options, allowing for a broader range of detection chemistries and assay multiplexing. The 96-well format has 6 excitation filters (450C680nm) and 6 emission filters (500C730nm), and the 384-well format has 5 excitation filters (450C650nm) and 5 emission filters (500C700nm).? ? ? ? ? ? ? ?Precise quantification with 1.5-fold discrimination AExcellent reproducibility and 10-log dynamic rangeMelt curve analysisGenotyping analysisBFigure 6. Real-time PCR reproducibility. This plot shows results from amplification of KAZ target plasmid DNA in 10-fold dilutions using the 96-well block. The data show highly reproducible results over 10 logs of input template amount, illustrating the broad linear dynamic range of the system.Figure 7. Melt curve analysis using the online version of the software. In this experiment, 96 replicates of human genomic DNA were amplified using SYBR Select Master Mix with primers for RNASE P followed by a dissociation step. The reactions were run under Fast run conditions, showing Ct uniformity with a mean of 25.7 (SD 0.077), and thermal uniformity as measured by the derivative peak with a melting temperature (Tm) of 84.17°C (SD 0.07°C).?Multiplex gene expressionCTFigure 9. Allelic discrimination plot with traces using real-time PCR data. Cluster plot of 44 gDNA samples and 4 no-template controls (NTCs) genotyped using TaqMan SNP Genotyping Assay C_, with both PCR and allelic discrimination performed on the QuantStudio 5 Real-Time PCR System. The novel use of realtime PCR data to plot SNP cluster progress aids in calling ambiguous samples and reduces run times by displaying the optimal number of cycles necessary for maximum cluster separation.?For more information about TaqMan Assays and formats, go to thermofisher.com/taqmanFigure 5. The QuantStudio 3 and 5 systems provide sensitive detection and highconfidence target discrimination down to 1.5-fold di?erences. (A) Amplification plots for 1.5-fold dilutions of a KAZ plasmid amplified with the PE2 TaqMan Assay under Fast run conditions using the TaqMan Fast Advanced Master Mix. Quantities assayed, and Ct (SD): 1,000 copies, 27.9 (0.063); 1,500 copies, 27.4 (0.059); 3,000 copies, 26.4 (0.060); 4,500 copies, 25.8 (0.047); 6,667 copies, 25.2 (0.049); 10,000 copies, 24.5 (0.041). NTC = no-template control. (B) Standard curve generated from the Ct values.? ?9QuantStudio 3 and 5 Real-Time PCR SystemsFigure 8. Multiplex reaction with 4 targets plus passive reference. Whole-plate amplification plots of 96 replicates of cDNA made from universal human RNA (UHR) amplified under Fast run conditions using TaqMan Multiplex Master Mix with MUSTANG PURPLE passive reference dye. Targets and labels: FZD1 labeled with FAM dye, APOE labeled with VIC dye, CD44 labeled with ABY dye, GAPDH labeled with JUN dye.? ? ?QuantStudio 3 and 5 Real-Time PCR Systems 10Technical specificationsQuantStudio 3Sample capacity (wells) Reaction volume 96 0.1mL block: 10C30 μL 0.2mL block: 10C100 μLQuantStudio 596 or 384 96-well: 0.1 mL block: 10C30 μL 0.2 mL block: 10C100 μL 384-well: 5C20 μL 27 cm x 50 cm x 40 cm Bright white LED 96 wells: 6 decoupled filters 384 wells: 5 coupled filters 96 wells: 450C680 nm/500C730 nm 384 wells: 450C650 nm/500C700 nm 96-well: up to 6 targets 384-well: up to 5 targets Optional Peltier 96 wells: 6 VeriFlex zones 384 wells: NA 6.5°C/sec 3.66°C/sec 0.4°C 0.25°C &30-minute runs?Footprint (W x D x H) Excitation source Optical detection Excitation/detection range Multiplexing 2D barcode reading Heating/cooling method Temperature zone function Max block ramp rate Average sample ramp rate Temperature uniformity Temperature accuracy Run time Dye compatibility (name)27 cm x 50 cm x 40 cm Bright white LED 4 coupled filters 450C600 nm/500C640 nm Up to 4 targets Optional Peltier 3 VeriFlex zones 6.5°C/sec 3.66°C/sec 0.4°C 0.25°C &30-minute runs FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/ NED/TAMRA/Cy 3, JUN, ROX/Texas Red?FAM/SYBR Green, VIC/JOE/HEX/TET, ABY/ NED/TAMRA/Cy3, JUN, ROX/Texas Red, MUSTANG PURPLE, Cy 5/LIZ , Cy 5.5? ? ?Chemistry capabilities Features to assist with 21 CFR Part 11 compliance Number of copies SensitivityFast/standard No 1 copy Detect di?erences as small as 1.5-fold in target quantities in singleplex reactionFast/standard Yes, with no additional fees 1 copy Detect di?erences as small as 1.5-fold in target quantities in singleplex reaction11 QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems 12Service and support to help meet your changing needsService plans at a glanceSmartStart orientation Every QuantStudio 3 and QuantStudio 5 system includes a SmartStart orientation to get you up and running quickly in your lab. The orientation includes basic qPCR familiarization and setup with both Thermo Fisher Cloud and online Instrument Management. QuantStudio 5 system owners receive a personalized qPCR application training. Online Instrument Management Sign in to your account to access the award-winning free online Instrument Management tool that enables faster responses to requests for service or service quotes, plus fast connection to key instrument and service information.? ?Comprehensive instrument warranty Our factory-trained and certified field service engineers (FSEs) are focused on delivering the highestquality workmanship. During the warranty period all qualifying repairs, including engineer time and travel, are covered.Flexible service plans Choose from a variety of service options that balance your budget, productivity, uptime, and regulatory requirements. Plans start with the most basic repair models and scale to premium offerings including advanced support and compliance services. On-site service plans are optimal for labs that have time-sensitive work and need to get their instrument back online quickly. These plans include guaranteed response times in most regions, scheduled planned maintenance, and automatic software updates. The AB Repair Center plan is a cost-effective choice for customers who can allow their instrument to be sent away for repair ― this plan provides a loaner instrument so that customers can maintain productivity while their instrument is being repaired. Compliance and validation services Our compliance and validation services are designed to help you balance business and regulatory requirements. From risk assessment and hardware/software qualification to full system validation, we partner with you to help mitigate regulatory risks and get your processes up and running.Training courses Our application and instrument training programs are led by scientists who aim to enhance your workday through experimental design best practices, workflow training, and instrument troubleshooting. Hands-on classes are available at our Thermo Fisher Scientific training centers or in your lab. Technical support If you have questions about product selection or use, assay or experiment design, data analysis, or troubleshooting, contact our team of technical support scientists or access our online product and application support tools. Financing options If you’re looking for accelerated return on investment, technology protection, or cash flow management, our innovative financing options can help meet your company’s budgetary needs and bottom-line goals. Contact your local sales representative for more details.Repair Center service plans Service plans at a glaceOn-site response time Scheduled on-site planned maintenance (PM) Remote diagnostics Parts, labor, and travel for repair, included Computer repair and replacement, included Priority access to Tech Support MonCFri, 8 a.m.C5 p.m. local time Priority access to Remote Service Engineer Requalification post-PM and critical repairs Field Application Scientist consultation Loaner instrument issued during repair (Repair Center plans only)On-site service plans AB AssuranceGuaranteed 2 business daysAB Repair Center AB Repair Center AB Maintenance Support Plus Support Plus Care PlusTarget 2 business days?AB CompleteGuaranteed next business day??? ? ? ? ? ? ?? ?10% discount optional add-on in selected regions? ? ? ?? ? ? ? ????? ? ???For a full schedule of courses, including self-paced online classes, go to thermofisher.com/training?2012 Oracle Fusion Middleware Innovation Award. ? Online Instrument Management tool not available in all regions.??Response times vary by region.13
QuantStudio 3 and 5 Real-Time PCR SystemsQuantStudio 3 and 5 Real-Time PCR Systems 14Ordering informationProduct QuantStudio 3 system configurations QuantStudio 3 Real-Time PCR System (96-well, 0.1 mL block) QuantStudio 3 Real-Time PCR System (96-well, 0.2 mL block)?Cat. No.A2 ZG11SCQS3STD A28480?QuantStudio 3 Real-Time PCR System AB Assurance Service Plan QuantStudio 3 Real-Time PCR System IQ/OQ/IPV ServiceQuantStudio 5 system configurations QuantStudio 5 Real-Time PCR System (96-well, 0.1 mL block) QuantStudio 5 Real-Time PCR System (96-well, 0.2 mL block) QuantStudio 5 Real-Time PCR System (384-well block)? ?A2 A28140 ZG11SCQS5STD A28482?QuantStudio 5 Real-Time PCR System AB Assurance Service Plan QuantStudio 5 Real-Time PCR System IQ/OQ/IPV Service?Does not include computer. Additional Cat. Nos. are available that include laptop or desktop computer.How to reach usTo find your order support or technical support team, go to thermofisher.com/contactus For product FAQs, protocols, training courses, and webinars, go to thermofisher.com/technicalresourcesFind out more at thermofisher.com/quantstudio3-5For Research Use Only. Not for use in diagnostic procedures. ? 2015 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified. TaqMan is a registered trademark of Roche Molecular Systems, Inc., used under permission and license. Mac is a trademark of Apple Inc. Amazon Web Services is a trademark of Amazon Technologies, Inc. Oracle is a trademark of Oracle International Corporation. CO5}