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Characterization of an O-desmethylangolensin-producing bacterium isolated from human fecesShin-ichiro YokoyamaToshio NiwaToshihiko OsawaTohru SuzukiOriginal PaperDOI:
10.-009-0524-5Yokoyama, S., Niwa, T., Osawa, T. et al. Arch Microbiol (: 15. doi:10.-009-0524-5
A bacterium that converted daidzein to O-desmethylangolensin was isolated from the feces of healthy humans. It was an obligately anaerobic, nonsporeforming, nonmotile and Gram-positive rod. The isolate used glucose, sucrose, raffinose, maltose, and fructose as carbon sources. It did not hydrolyze gelatin, esculin, or starch. The strain was urease, acid phosphatase, and arginine dihydrolase positive. It was catalase, oxidase, H2S, and indole negative. The major products of glucose fermentation were butyrate and lactate. Its mol% G+C was 51.2. The major cellular fatty acids were C16:0 DMA, C16:0, and C16:0 aldehyde. The structural type of cell wall peptidoglycan was suggested to be A1γ. The isolate was susceptible to β-lactam, cefem, and macrolide antibiotics and resistant to aminoglycoside and quinolone antibiotics. The bacterium was related to Eubacterium ramulus ATCC29099T, Eubacterium rectale ATCC33656T, and species of the genus Roseburia, but the highest 16S rRNA gene similarity to these described species was only 94.4%, consistent with its being classified as a novel genus. Based on the above, the isolate, named strain SY8519, was identified as belonging to a novel genus in the Clostridium rRNA cluster XIVa.O-desmethylangolensin Strain SY8519 Isolate from human feces Taxonomical property Clostridium rRNA cluster XIVa Antimicrobial susceptibility Akaza H, Miyanaga N, Takashima N, Naito S, Hirano Y, Tsukamoto T, Mori M (2002) Is daidzein non-metabolizer a high risk for prostate cancer? A case-controlled study of serum soybean isoflavone concentration. Jpn J Clin Oncol 32:296–300Altschul SF, Madden TL, Schaffer AA, Zhang JH, Zhang Z, Miller W, Lipman DJ (1997) Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acids Res 25:Arai Y, Uehara M, Sato Y, Kimira M, Eboshida A, Adlercreutz H, Watanabe S (2000) Comparison of isoflavones among dietary intake, plasma concentration and urinary excretion for accurate estimation of phytoestrogen intake. J Epidemiol 10:127–135Braune A, Gütschow M, Engst W, Blaut M (2001) Degradation of quercetin and luteolin by Eubacterium ramulus. Appl Environ Microbiol 67:Brienholt V, Larsen JC (1998) Detection of weak estrogenic flavonoids using a recombinant yeast strain and a modified MCF7 cell proliferation assay. Chem Res Toxicol 11:622–629Collins MD, Lawson PA, Willems A, Cordoba JJ, Fernandez-Garayzabal J, Garcia P, Cai J, Hippe H, Farrow JAE (1994) The phylogeny of the genus Clostridium: proposal of five new genera and eleven new species combinations. Int J Syst Bacteriol 44:812–826Downes J, Munson MA, Radford DR, Spratt DA, Wade WG (2002) Shuttleworthia satelles gen. nov., sp. nov., isolated from human oral cavity. Int J Syst Evol Microbiol 52:Duncan SH, Flint HJ (2008) Proposal of a neotype strain (A1–86) for Eubacterium rectale. Request for an opinion. Int J Syst Evol Microbiol 58:Duncan AM, Merz-Demlow BE, Xu X, Phipps WR, Kurzer MS (2000) Premenopausal equol excretors show plasma hormone profiles associated with lowered risk of breast cancer. Cancer Epidemiol Biomarkers Prev 9:581–586Duncan SH, Hold GL, Barcenilla A, Stewart CS, Flint HJ (2002) Roseburia intestinalis sp. nov., a novel saccharolytic, butyrate-producing bacterium from human faeces. Int J Syst Evol Microbiol 52:Duncan SH, Aminov RI, Scott KP, Louis P, Stanton TB, Flint HJ (2006) Proposal Roseburia faecis sp. nov., Roseburia hominis sp. nov.,
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Biosci Biotechnol Biochem 72:Shin-ichiro Yokoyama12Toshio Niwa3Toshihiko Osawa3Tohru Suzuki41.Department of Applied MicrobiologyGifu Prefectural Research Institute for BioengineeringGifuJapan2.Regional R&D Promotion Division Science and Technology Policy BureauMinistry of Education, Culture, Sports, Science and Technology-JapanTokyoJapan3.Laboratory of Food and BiodynamicsNagoya University Graduate School of Bioagricultural SciencesNagoyaJapan4.The United Graduate School of Agricultural Science, Gifu UniversityGifuJapan
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