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&& &&& 常用流式细胞仪荧光参数
小白讲实验，带你走进科研殿堂，助你从实验小白变身科研达人实验小白做流式实验之前首先要了解您用的流式细胞仪能检测哪些荧光染料，然后才能配色，最后购买荧光抗体进行实验。目前，临床和科研中的使用的主要流式细胞仪品牌包括BD Biosciences、Beckman Coulter、 Partec、Miltenyi Biotec、Life Technologies以及 EMD Millipore等，其中BD Biosciences和Beckman Coulter这两大品牌的流式细胞仪占据了国内绝大多数市场。因此，实验小白这一期为大家分享这两大品牌常用型号的流式细胞仪的荧光参数，供大家参考！BD BiosciencesBD FACSCalibur(TM)&2-laser,&4-color&FL1: FITC, BD Horizon Brilliant Blue 515, Alexa Fluor 488, CFSE, GFP ;FL2: PE, PI;FL3: PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy7, PI, 7-AAD, PI, PerCP-eFluor(R) 710;FL4: APC, Alexa Fluor 647.BD Accuri(TM) C6&2-laser,&4-colorFL1: FITC, BD Horizon Brilliant Blue 515, Alexa Fluor 488, CFSE, GFP, YFP ;FL2: PE, PI, PE-CF594, YFP;FL3: PerCP, PE-Cy5, PerCP-Cy5.5, PE-Cy7, PI, 7-AAD, RFP, PI, PE-CF594, PerCP-eFluor(R) 710;FL4: APC, Alexa Fluor 647.&BD FACSVerse(TM)3-laser, 8-color (4-2-2) configurationFL1: FITC, BD Horizon Brilliant Blue 515, Alexa Fluor 488, CFSE, GFP;FL2: PE, PI ;FL3: PerCP, PerCP-Cy5.5, PE-Cy5, 7-AAD, PerCP-eFluor(R) 710;FL4: PE-Cy7;FL5: APC, Alexa Fluor 647;FL6: APC-Cy7, APC-H7, APC-eFluor(R) 780, APC-Fire(TM) 750;FL7: BD Horizon(TM) V450, Pacific Blue(TM), DAPI BD Horizon(TM) Violet Proliferation Dye 450, BD Horizon(TM) Fixable Viability Stain 450, BD Horizon Brilliant(TM) Violet 421, eFluor(R) 450;FL8: BD Horizon(TM) V500, BD Horizon Brilliant(TM) Violet 510, AmCya.BD FACSCanto(TM) II &3-laser, 4-3-3 configurationFL1: FITC, Alexa Fluor 488;FL2: PE, PI, PE-Texas Red(R), eFluor(R) 605NC ;FL3: PerCP, PE-Cy5, 7-AAD, PerCP-Cy(TM)5.5, PerCP-eFluor(R) 710;FL4: PE-Cy7;FL5: APC, Alexa Fluor 647;FL6: Alexa 700;FL7: APC-Cy7, APC-H7, APC-eFluor(R) 780, APC-Fire(TM) 750;FL8: BD Horizon(TM) V450, BD Horizon Brilliant(TM) Violet 421, eFluor(R) 450;FL9: BD Horizon(TM) V500-C, AmCyan, BD Horizon Brilliant(TM) Violet 510;FL10: BD Horizon Brilliant(TM) Violet 605.BD FACSAria(TM) III or Fusion&6-laser, 18-colorFL1: DAPI, Hoechst BFL2: Hoechst RFL3: BD Horizon Brilliant(TM) Violet 421, BD Horizon(TM) V450, BD Horizon(TM) VPD450, Pacific Blue(TM), DAPI, eFluor(R) 450;FL4: BD Horizon Brilliant(TM) Violet 510, BD Horizon(TM) V500, AmCFL5: BD Horizon Brilliant(TM) Violet 605;FL6: BD Horizon Brilliant(TM) Violet 650;FL7: BD Horizon Brilliant(TM) Violet 711;FL8: BD Horizon Brilliant(TM) Violet 786;FL9: CFP, AmCyanFL10: FITC, BD Horizon Brilliant(TM) Blue 515, Alexa Fluor(R) 488;FL11: PerCP, PerCP-Cy(TM)5.5, PerCP-eFluor(R) 710;FL12: PE, DsRFL13: PE-Texas Red(R), mCherry, PI, eFluor(R) 605NC;FL14: PE-Cy(TM)5, PE-Cy5.5;FL15: PE-Cy7;FL16: APC, Alexa Fluor(R) 647;FL17: Alexa Fluor(R) 700;FL18: APC-Cy7, APC-H7, APC-eFluor(R) 780, APC-Fire(TM) 750.Beckman CoulterFC500&1-laser, 5-colorFL1: FITC;FL2: PE;FL3: PE-Texas Red (ECD), eFluor(R) 605NC;FL4: PE-Cy5 (PC5), PerCP-eFluor(R) 710;FL5: PE-Cy7 (PC7);2-laser, 5-colorFL1: FITC;FL2: PE;FL3: PE-Texas Red (ECD), eFluor(R) 605NC;FL4: PE-Cy7 (PC7);FL5: APC, APC-Cy7.PS：不同颜色的字体代表这些染料所用的激发光的颜色（355nm, 405nm, 445nm, 488nm, 561nm, 633nm）。我们所列举的都是每种型号的流式细胞仪常规配置时所能有的染料组合。实际上，每台流式细胞仪所能用的染料组合取决于这台仪器实际的硬件配置（Lasers, filters, Mirrors）。换句话说，即使是同样型号的流式细胞仪，由于其配置不同，所能检测的荧光组合也不相同。另外，值得注意的是，BD FACSAria(TM) III or Fusion型号的流式细胞仪如果安装了561nm的绿色激光，那么488nm的蓝色激光仅仅用于检测FITC和PerCP、PerCP-Cy(TM)5.5这两大类型的荧光。而PE、PE-Texas Red、PE-Cy(TM)5、PE-Cy5.5、PE-Cy7这些染料则是通过561nm的绿色激光激发后检测的。这样的设计可以有效的减少FITC和PE间的补偿。当然，如果BD FACSAria(TM) III or Fusion型号的流式细胞仪没有安装561nm的绿色激光，那么488nm的蓝色激光也可以用于激发FITC、PerCP、PerCP-Cy(TM)5.5以及PE、PE-Texas Red、PE-Cy(TM)5、PE-Cy5.5、PE-Cy7等染料。欢迎个人转发分享，刊物和机构如需转载，请联系授权事宜：实验小白任何稳定性高、重复性好的实验技术实例都可与我们分享。我们也真诚邀请您撰写某一领域的前沿和今后的发展方向，为大家的课题设计指明方向！投稿通道：1.邮箱:.2.添加微信号:Cjer0201.3.添加qq号:.PS: 添加qq群号: （实验小白），下载相关资源。点击下方“阅读原文”查看更多精彩内容
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BD FACSalibur流式细胞仪操作手册.doc27页
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BD FACSCalibur流式细胞仪
FACS101 Handbook
本课程介绍「表面抗原流式分析」有关之基础工作原理。如希望进一步了解流式细胞技术应用，请至本公司网站订阅FACSinformation电子报.tw/bdb/index.html。
如需要本课程手册，欢迎至本公司网站下载 .tw/bdb/10-5-3-1.html 。
如需要免疫荧光染色方法，请至本公司网站下载 .tw/bdb/10-5-4-1.html 。
一、BD FACSCalibur基本结构
1.1仪器本体： 1. 电源开关：在BD FACSCalibur仪器右侧下方，先启动仪器本体，再打开计算机。 2. 光学系统：BD FACSCalibur 基本配有一支波长488 nm 的氩离子雷射
以BD FACSCalibur 基本型为例
FSC Diode 只收488 nm波长散射光
SSC PMT 只收488 nm波长散射光
FL1 PMT 荧光光谱峰值落在绿色范围（波长515-545 nm）
FL2 PMT 荧光光谱峰值落在橙红色范围（波长564-606 nm）
FL3 PMT 荧光光谱峰值落在深红色范围（波长
650 nm） 3. 仪器面板：
仪器前方面板的右下方有三个流速控制键、及三个功能控制键。
流速控制：
样品流速：12
MED：样品流速：35
HI: 样品流速：60
l /min 功能控制：
RUN：此时上样管加压，使细胞悬液从进样针进入流动室。（正常显示绿色；黄色时表示仪器不正常，请检查是否失压。）
STANDBY： 无样品或暖机时之正常位置，此时鞘液停止流动，雷射功率自动降低。
PRIME：去除流动室中的气泡，流动室施以反向压力，将液流从流动室冲入样品管，持续一定时间后，以鞘液回注满流动室。PRIME 结束，仪器恢复STANDBY状态。 4. 储液箱抽屉：
在主机左下方之储液箱抽屉。可向前拉开，内含鞘流液筒、废液筒、鞘液过滤器Sheath Filter，及空气滤网 Air filter。请注意气路减压阀VENT TOGGLE之位置。 鞘液筒：位于抽屉左侧，容积4升。装
正在加载中，请稍后...&&&流式细胞技术 在 消化系统疾病 分类中
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&&&&To investigate the relationship between the efficacy of interferon-α and CD28
+ PBMCs in patients with chronic hepatitis B. CD28
+PBMCs in 32 patients and 8 healthy persons were detected by indirectly immunofluorescent flow cytometry technique.
&&&&探讨干扰素疗效与慢性乙肝患者CD2 8阳性外周血单个核细胞 (PBMCs)的关系。 分离 32例慢性乙肝患者及 8例正常对照PBMCs,以间接免疫荧光流式细胞技术检测CD2 8+ PBMCs,结果显示慢性乙肝患者在干扰素治疗前和结束时CD2 8+ PBMCs均较正常对照组显著降低 (分别为P <0
&&&&Methods The serum levels of soluble TRAIL(sTRAIL), IFN-γ and membrane bound TRAIL expression on peripheral leucocytes from 58 CHB patients were examined by ELISA and flow cytometry respectively.
&&&&方法 采用流式细胞技术及夹心酶联免疫吸附法分析58例慢性肝炎患者外周血CD_4~+、CD_8~+ T淋巴细胞膜TRAIL及血清可溶性TRAIL(sTRAIL)表达水平,同时检测血清γ干扰素(IFN-γ)水平变化并与肝功能相关指标进行相关性分析。
&&&&Objective To discuss the relationship between the substype of peripheral blood CD8 lymphocyte in patients with chronic hepatitis virus B and clinic condition and the replication of HBV. Methods CD45RO, CD45RA and CD28 of peripheral blood CD8 lymphocyte were detected by flow cytometry with multicolor fluorescence technology in 107 patients with chronic hepatitis virus B and 20 healthy volunteers.
&&&&目的 探讨慢性乙型肝炎患者外周血CD8淋巴细胞亚型与其临床状态及HBV复制的关系。 方法 采用流式细胞技术多色荧光分析法,检测了18名健康体检者和107例慢性乙型肝炎患者外周血CD8淋巴细胞CD45R0、CD45RA和CD28的表达及病毒载量检测。
&&&&Methods The levels of CD28 on peripheral T lymphocyts in forty patients and thirty normal persons were detected by flow cytometry(FCM).
&&&&方法采用流式细胞技术(FCM)用三色标记法检测40例丙肝患者和30例正常人群外周血T细胞CD28的表达。
&&&&Methods The peripheral blood were collected from 104 patients with chronic hepatitis B and 30 healthy individuals,then CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+ and CD8+CD45RO+ T lymphocyte subsets were detected by flow cytometry with three-color fluorescence technology.
&&&&方法采集46例轻中度慢性乙型肝炎患者、58例重度慢性乙型肝炎患者和30例健康人的外周抗凝血,应用流式细胞技术三色荧光分析法对其外周血中CD4+CD45RA+,CD4+CD45RO+,CD8+CD45RA+和CD8+CD45RO+T淋巴细胞亚群进行检测.
&&&&The T lymphocyte subset of peripheral blood was analyzed by FCM before and after treatment for 12 weeks and 24 weeks . And in a long time we assessed the HBV markers, HBV-DNA and alanine aminotransferase (ALT) levels. The data were analyzed with SPSS software.
&&&&采用荧光定量PCR法检测患者的HBV基因型，流式细胞技术检测患者抗病毒前及抗病毒后12周、24周的外周血T细胞亚群，并随访服药48周后的HBV标志物、HBV-DNA定量以及肝功能。
&&&&By means of FCM the effects of calcium antagonists,tetrendrine(Tet),nifedipine (Nif), verapamil(Ver) and cinnacine(Cin),with different doses, on growth and proliferation of 3T6 fibroblasts were observed, The results showed that they could block the process of Gl→S phase,reduce DNA contents of S phase,as well as increase protein contents of G1 and G2 phases,the growth of cells lost its balance,then death occurred finally.
&&&&采用流式细胞技术观察不同剂量的四种钙拮抗剂（Ca－A）汉防己甲素（Tet）、硝苯啶（Nif）、维拉帕咪（Ver）及脑益嗪（Cin）对3T6成纤维细胞生长增殖的影响。 结果表明它们不仅可阻断Gl→S期的进程，而且抑制S期DNA的含量，但却增加Gl和G2期蛋白质的含量，使细胞呈现不平衡生长，最终死亡。
&&&&The proliferation of HSC was measured by
3H-thymidine incorporation,and the collagen synthesis by
3H-Proline. The changes of HSC cycle were analyzed by FCM. Results At dose of 10
-4 mol/L,ALD increased the incorporation of
3H-TdR and
3H-Pro( P <0.05),but didn′t at the lower doses.
&&&&方法　大鼠HSC培养 ,在不同浓度的ALD、螺内酯作用下 ,采用3 H 胸腺嘧啶核苷 (3 H TdR)、3 H 脯氨酸 (3 H Pro)掺入法及流式细胞技术观察ALD、螺内酯对HSC增殖及胶原合成的影响。
&&&&Methods:Peripheral Tlymphocyte actication was tested by flowcytomety(FCM)in187cases of HBV infected patients and16healthy subjects,while the HBV-DNA load was detected by real time fluorescence PCR in the same samples.
&&&&方法 :应用流式细胞技术检测187例乙型肝炎患者及16例健康对照外周血中淋巴细胞的活化 ,同时利用实时荧光定量PCR测定其体内HBV -DNA载量。
&&&&Methods ELISA and FCM.
&&&&方法　EL ISA与流式细胞术。
FCM (flow cytometry) :PBMCs were fixed by 10% ethanol at -30T!
&&&&2.流式细胞术(flow eytome坷，FCM):
&&&&apoptosis were examined by flow cytometry (FCM) and DNA
&&&&用DNA凝胶电泳及流式细胞仪检测细胞凋亡 ;
&&&&The expression of caspase-3 protein was detected by FCM.
&&&&应用流式细胞方法测定caspase - 3蛋白表达。
&&&&Cells proliferation was detected with MTT colorimetric assay. Cells apoptosis was detected by flow cytometry.
&&&&MTT比色法测定细胞增殖,流式细胞仪检测细胞凋亡。
查询“流式细胞技术”译词为用户自定义的双语例句&&&&我想查看译文中含有：的双语例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法，我们为您准备了出自英文原文的大量英语例句，供您参考。&&&&&&&&&&&& By means of FCM the effects of calcium antagonists,tetrendrine(Tet),nifedipine (Nif), verapamil(Ver) and cinnacine(Cin),with different doses, on growth and proliferation of 3T6 fibroblasts were observed, The results showed that they could block the process of Gl→S phase,reduce DNA contents of S phase,as well as increase protein contents of G1 and G2 phases,the growth of cells lost its balance,then death occurred finally.Among these 4 calcium antagonists,Tet was one of the most prominant effect,and was related... By means of FCM the effects of calcium antagonists,tetrendrine(Tet),nifedipine (Nif), verapamil(Ver) and cinnacine(Cin),with different doses, on growth and proliferation of 3T6 fibroblasts were observed, The results showed that they could block the process of Gl→S phase,reduce DNA contents of S phase,as well as increase protein contents of G1 and G2 phases,the growth of cells lost its balance,then death occurred finally.Among these 4 calcium antagonists,Tet was one of the most prominant effect,and was related to the dosage.The conclusion is that the inhibition of growth and proliferation to 3T6 fibroblasts by calcium antagonists is probably one of the factors of the mechanism of their ability to anti-liver fibrosis.采用流式细胞技术观察不同剂量的四种钙拮抗剂（Ｃａ－Ａ）汉防己甲素（Ｔｅｔ）、硝苯啶（Ｎｉｆ）、维拉帕咪（Ｖｅｒ）及脑益嗪（Ｃｉｎ）对３Ｔ６成纤维细胞生长增殖的影响。结果表明它们不仅可阻断Ｇｌ→Ｓ期的进程，而且抑制Ｓ期ＤＮＡ的含量，但却增加Ｇｌ和Ｇ２期蛋白质的含量，使细胞呈现不平衡生长，最终死亡。其中Ｌｅｔ的作用最为显著，呈一定的量效关系，此作用与阻断钙离子内流无关。提示钙拮抗剂对３Ｔ６成纤维细胞生长增殖的抑制可能是其抗肝纤维化的机制之一。 The effects of calcium antagonists, tetrendrine(Tet), nifedipine(Nif), verapamil(Ver) and cinnacine(Cin) 3T6 fibroblasts, were observed by FCM. The results showed that they can not only block G1→S phase, but also reduce DNA content of S phase, increase protein content of G1 and G2 phases. 3T6 cells will die as soon as possible. In all of the calcium antagonists, Tet has the most remarkable actions and showes the relationship between doses and effects. We conclude that calcium antagonists can inhibit growth and... The effects of calcium antagonists, tetrendrine(Tet), nifedipine(Nif), verapamil(Ver) and cinnacine(Cin) 3T6 fibroblasts, were observed by FCM. The results showed that they can not only block G1→S phase, but also reduce DNA content of S phase, increase protein content of G1 and G2 phases. 3T6 cells will die as soon as possible. In all of the calcium antagonists, Tet has the most remarkable actions and showes the relationship between doses and effects. We conclude that calcium antagonists can inhibit growth and proliferation of 3T6 fibroblasts, and this may be one of its antiliver fibrosis mechanisms.采用流式细胞技术（ＦＣＭ）观察不同剂量的四种钙拮抗剂（Ｃａ－Ａ）汉防已甲素（Ｔｅｔ）、硝苯啶（Ｎｉｆ）、维拉帕咪（Ｖｅｒ）及脑益嗪（Ｃｉｎ）对３Ｔ６成纤维细胞生长增殖的影响，结果表明它们不仅可阻断Ｇ１→Ｓ期的进程，而且抑制Ｓ期ＤＮＡ的含量，但却增加Ｇ１和Ｇ２期蛋白质的含量，使细胞呈现不平衡生长，最终死亡。其中Ｔｅｔ的作用最为显著，呈一定的量效关系，此作用与阻断钙离子内流无关。提示钙拮抗剂对３Ｔ６成纤维细胞生长增殖的抑制可能是其抗肝纤维化的机制之一。 Objective To study the role of tokicyte T cell in active hepatitis. Methods We examined CD28+T cell in patientS with chronic active/remission hepatitis by flow cytometry. Results it showsthat CD28+, CDS+CD28+T cell in patients with chronic active tiepatitis decreased significantly, comparedwith normal control or patients with liver cirrhosis and heptoma. Conclusion it implies that B7-CD28costimulation pathway contribute to the tissue injury in hepatitis. 目的了解肝病患者中细胞毒性Ｔ细胞异常免疫激活的机理。方法采用流式细胞技术检测了不同类型肝病患者外周血单个核细胞（ｐｅｒｉｐｈｅｒａｌｂｌｏｏｄｍｏｎｏｎｕｃｌｅａｒＣｅｌｌ，ＰＢＭＣ）中Ｔ淋巴细胞亚群的共刺激分子ＣＤ２８的表达情况。结果慢性肝炎活动期患者中ＣＤ２８＋，ＣＤＳ＋ＣＤ２８＋Ｔ细胞比正常对照组显著下降，而肝硬化、肝癌及慢性肝炎缓解期组与正常对照组无显著差异。结论提示Ｂ７—ＣＤ２８共刺激在慢性肝炎活动期的肝损伤中起作用。&nbsp&&&&&相关查询
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