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? (Tyner lab)This is a basic immunohistochemistry protocol. Protocols for immunohistochemistry usr/localy widely, due to the differences between antigens and their recognition by antibody. For success it is crucial to try a number of different techniques and compare the results.&
(KPL)General Protocol for staining thick sections&&
(LEP)This staining procedure is for tissues fixed in 10% neutral buffered formalin and embedded in paraffin&&&&&&
(Nottingham Pathology Lab)&&
(R&D)General protocol including details for sample preparation, tissue staining and trouble shooting&&
(R&D)General protocol&&&
(eBioscience) &&&
(Karl Clark)Immunohistochemistry allows visualization of antigens (usually proteins) within an embryo. Typically, a primary antibody binds specifically to an antigen (e.g. Luciferase); then a secondary antibody conjugated to a flourochrome or enzyme is bound to the heavy chain constant region of the primary antibody with specificity to the species of origin of the primary (e.g. anti-rabbit conjugated to Rhodamine&&
(Vesicle Trafficking)&&
(Karl Clark)Beta-gal staining allows identification of embryonic tissues/cells expressing lacZ marker protein by development of pigmented (blue) product in the presence of lacZ enzymatic activity.&
(Cepko Lab)Very detailed protocol with background information and several applications.&&
(House Ear Institute)A recipe for Moviol,an inexpensive mounting medium that is easy to prepare.&&
Energy Beam Sciences, Inc.)Introduction to microwave immunohistochemistry technique and its applicaiton protocol&&&
(Mike A. Dyer)&&
(Mike A. Dyer)&
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Eppendorf 荧光定量 PCR仪
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