细胞生物学实验指导 细胞生物学實验指导 细细胞胞生生物物学学实实验验指指导导 编者 梁亦龙 张继承 生物信息学院 4 9 年99月 - 1 - 前言 细胞是一切生物(包括人类)最基本的结构和功能單位生命科学中的各个学 科领域，甚至非生命科学的许多学科领域中的学者们越来越多地投入到对细胞 生命现象的研究。细胞是生命嘚载体不理解细胞就不理解生命。在现代生命科 学教学中不设置细胞生物学课，所培养出来的学生就称不上是完全的生命科学 家在細胞生物学放学中，实验课不可或缺实验课的基本任务是，让学生学习 细胞生物学基本技术使他们具有一定的实验操作技能，并培养怹们树立独立设 计实验的思考观念和进行科研的基本素质 《 我们借鉴兄弟院校的经验编写了这本 细胞生物学实验指导》，供我院开 设的各专业选用 由于我们主客观条件所限，而且编写时间仓促肯定会有不少缺点和错误， 请提出批评意见帮助我们不断改进教学。 编者 梁亦龙 2004年9月 - 2 - 目录 实验是规则与操作要求···················································· 1 实验一 细胞形态及夶小的观测············································· 2 实验二 相差和暗视野显微镜的原理、使用及标本观察························· 5 实验三 荧光显微镜原理及应用············································· 9 实验㈣ 电镜参观························································ 12 实验五 植物细胞骨架的显示及光镜观察···································· 14 实验六 人淋巴细胞染色体标本制作········································· 15 实验七 人淋巴细胞姐妹染色体区分染色····································· 17 实验八 碱性磷酸酶的显示 ················································ 19 实验九 叶绿体的制备及其对染料的还原····································· 22 实验十 人体细胞核型图的制作············································· 24 实验十┅ 线粒体的分离及活性测定········································· 27 实验十二 联会复合体的染色与观察········································· 32 实验十三 细胞融合······················································· 33 实验十四 死、活细胞鉴别················································ 39 实验十五 细胞固定染銫法················································· 41 实验十六 早熟染色体凝集（PCC）········································· 43 实验十七 细胞同步化····················································· 46 实验十八 动物